chip wash buffer Search Results


chip licl immune complex wash buffer-i  (Boston BioProducts)
96
Boston BioProducts chip licl immune complex wash buffer-i
Chip Licl Immune Complex Wash Buffer I, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chip licl immune complex wash buffer-i/product/Boston BioProducts
Average 96 stars, based on 1 article reviews
chip licl immune complex wash buffer-i - by Bioz Stars, 2026-03
96/100 stars
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chip wash buffer  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology chip wash buffer
( a ) Illustration of experimental approach tracing the effect of developmental loss of subunit POLR3G, subunit-specific disruption of POLR3G, and cancer-associated re-establishment of POLR3G and <t>accompanying</t> <t>chromatin</t> features, which collectively identify POLR3G-driven modulation of Pol III transcription potential in proliferating cells. ( b ) Pol III subunit and transcription factor legend corresponding to genome-wide maps in panels c-k, including POLR3A/RPC1 (3A); POLR3B/RPC2 (3B); POLR1D/RPAC2 (1D); POLR3C/RPC3 (3C); POLR3G/RPC7α (3G); POLR3GL/RPC7β (3GL); POLR3D/RPC4 (3D); POLR3E/RPC5 (3E); BRF1/TFIIIB90; TF3C1/TFIIIC220. Corresponding subcomplex indicated. ( c-k ) Example <t>ChIP-seq</t> read signals for each subunit/factor are shown in THP-1 monocytes across canonical Pol III-transcribed genes of varying promoter architecture, including ribosomal 5S rRNA genes (type 1 promoter; panel c ), tRNA, 7SL, vault, and SNAR RNA genes (type 2 promoter, panels d-g , respectively), and Y, U6, 7SK, and RMRP RNA genes (type 3 promoter, panels h-k , respectively). Gene labels include Unique RNA Sequence (URS) identifiers assigned by (and connected to) the RNAcentral database. Bottom panel illustrates corresponding promoter architecture classification. ( l ) Cartoon schematic of the Pol III protein complex with emphasis on subunits mapped in this study (labeled) and the corresponding color code for genomic signal plots. Illustration serves as general reference guide inspired by previous structural reconstructions (Hoffmann et al., Nature 2015) . ( m ) Scatterplot visualization of the correlation between normalized read densities for paralogous Pol III subunits, POLR3G and POLR3GL, at Pol III complex-occupied genes in THP-1 monocytes.
Chip Wash Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chip wash buffer/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
chip wash buffer - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
diamag beads wash buffer (1x chip dilution buffer + 0.1% bsa)  (DIAGENODE DIAGNOSTICS)
90
DIAGENODE DIAGNOSTICS diamag beads wash buffer (1x chip dilution buffer + 0.1% bsa)
( a ) Illustration of experimental approach tracing the effect of developmental loss of subunit POLR3G, subunit-specific disruption of POLR3G, and cancer-associated re-establishment of POLR3G and <t>accompanying</t> <t>chromatin</t> features, which collectively identify POLR3G-driven modulation of Pol III transcription potential in proliferating cells. ( b ) Pol III subunit and transcription factor legend corresponding to genome-wide maps in panels c-k, including POLR3A/RPC1 (3A); POLR3B/RPC2 (3B); POLR1D/RPAC2 (1D); POLR3C/RPC3 (3C); POLR3G/RPC7α (3G); POLR3GL/RPC7β (3GL); POLR3D/RPC4 (3D); POLR3E/RPC5 (3E); BRF1/TFIIIB90; TF3C1/TFIIIC220. Corresponding subcomplex indicated. ( c-k ) Example <t>ChIP-seq</t> read signals for each subunit/factor are shown in THP-1 monocytes across canonical Pol III-transcribed genes of varying promoter architecture, including ribosomal 5S rRNA genes (type 1 promoter; panel c ), tRNA, 7SL, vault, and SNAR RNA genes (type 2 promoter, panels d-g , respectively), and Y, U6, 7SK, and RMRP RNA genes (type 3 promoter, panels h-k , respectively). Gene labels include Unique RNA Sequence (URS) identifiers assigned by (and connected to) the RNAcentral database. Bottom panel illustrates corresponding promoter architecture classification. ( l ) Cartoon schematic of the Pol III protein complex with emphasis on subunits mapped in this study (labeled) and the corresponding color code for genomic signal plots. Illustration serves as general reference guide inspired by previous structural reconstructions (Hoffmann et al., Nature 2015) . ( m ) Scatterplot visualization of the correlation between normalized read densities for paralogous Pol III subunits, POLR3G and POLR3GL, at Pol III complex-occupied genes in THP-1 monocytes.
Diamag Beads Wash Buffer (1x Chip Dilution Buffer + 0.1% Bsa), supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/diamag beads wash buffer (1x chip dilution buffer + 0.1% bsa)/product/DIAGENODE DIAGNOSTICS
Average 90 stars, based on 1 article reviews
diamag beads wash buffer (1x chip dilution buffer + 0.1% bsa) - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
chip immune complex wash buffer  (Boston BioProducts)
96
Boston BioProducts chip immune complex wash buffer
( a ) Illustration of experimental approach tracing the effect of developmental loss of subunit POLR3G, subunit-specific disruption of POLR3G, and cancer-associated re-establishment of POLR3G and <t>accompanying</t> <t>chromatin</t> features, which collectively identify POLR3G-driven modulation of Pol III transcription potential in proliferating cells. ( b ) Pol III subunit and transcription factor legend corresponding to genome-wide maps in panels c-k, including POLR3A/RPC1 (3A); POLR3B/RPC2 (3B); POLR1D/RPAC2 (1D); POLR3C/RPC3 (3C); POLR3G/RPC7α (3G); POLR3GL/RPC7β (3GL); POLR3D/RPC4 (3D); POLR3E/RPC5 (3E); BRF1/TFIIIB90; TF3C1/TFIIIC220. Corresponding subcomplex indicated. ( c-k ) Example <t>ChIP-seq</t> read signals for each subunit/factor are shown in THP-1 monocytes across canonical Pol III-transcribed genes of varying promoter architecture, including ribosomal 5S rRNA genes (type 1 promoter; panel c ), tRNA, 7SL, vault, and SNAR RNA genes (type 2 promoter, panels d-g , respectively), and Y, U6, 7SK, and RMRP RNA genes (type 3 promoter, panels h-k , respectively). Gene labels include Unique RNA Sequence (URS) identifiers assigned by (and connected to) the RNAcentral database. Bottom panel illustrates corresponding promoter architecture classification. ( l ) Cartoon schematic of the Pol III protein complex with emphasis on subunits mapped in this study (labeled) and the corresponding color code for genomic signal plots. Illustration serves as general reference guide inspired by previous structural reconstructions (Hoffmann et al., Nature 2015) . ( m ) Scatterplot visualization of the correlation between normalized read densities for paralogous Pol III subunits, POLR3G and POLR3GL, at Pol III complex-occupied genes in THP-1 monocytes.
Chip Immune Complex Wash Buffer, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chip immune complex wash buffer/product/Boston BioProducts
Average 96 stars, based on 1 article reviews
chip immune complex wash buffer - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier

Image Search Results


( a ) Illustration of experimental approach tracing the effect of developmental loss of subunit POLR3G, subunit-specific disruption of POLR3G, and cancer-associated re-establishment of POLR3G and accompanying chromatin features, which collectively identify POLR3G-driven modulation of Pol III transcription potential in proliferating cells. ( b ) Pol III subunit and transcription factor legend corresponding to genome-wide maps in panels c-k, including POLR3A/RPC1 (3A); POLR3B/RPC2 (3B); POLR1D/RPAC2 (1D); POLR3C/RPC3 (3C); POLR3G/RPC7α (3G); POLR3GL/RPC7β (3GL); POLR3D/RPC4 (3D); POLR3E/RPC5 (3E); BRF1/TFIIIB90; TF3C1/TFIIIC220. Corresponding subcomplex indicated. ( c-k ) Example ChIP-seq read signals for each subunit/factor are shown in THP-1 monocytes across canonical Pol III-transcribed genes of varying promoter architecture, including ribosomal 5S rRNA genes (type 1 promoter; panel c ), tRNA, 7SL, vault, and SNAR RNA genes (type 2 promoter, panels d-g , respectively), and Y, U6, 7SK, and RMRP RNA genes (type 3 promoter, panels h-k , respectively). Gene labels include Unique RNA Sequence (URS) identifiers assigned by (and connected to) the RNAcentral database. Bottom panel illustrates corresponding promoter architecture classification. ( l ) Cartoon schematic of the Pol III protein complex with emphasis on subunits mapped in this study (labeled) and the corresponding color code for genomic signal plots. Illustration serves as general reference guide inspired by previous structural reconstructions (Hoffmann et al., Nature 2015) . ( m ) Scatterplot visualization of the correlation between normalized read densities for paralogous Pol III subunits, POLR3G and POLR3GL, at Pol III complex-occupied genes in THP-1 monocytes.

Journal: bioRxiv

Article Title: A cancer-associated RNA polymerase III identity drives robust transcription and expression of SNAR-A noncoding RNA

doi: 10.1101/2021.04.21.440535

Figure Lengend Snippet: ( a ) Illustration of experimental approach tracing the effect of developmental loss of subunit POLR3G, subunit-specific disruption of POLR3G, and cancer-associated re-establishment of POLR3G and accompanying chromatin features, which collectively identify POLR3G-driven modulation of Pol III transcription potential in proliferating cells. ( b ) Pol III subunit and transcription factor legend corresponding to genome-wide maps in panels c-k, including POLR3A/RPC1 (3A); POLR3B/RPC2 (3B); POLR1D/RPAC2 (1D); POLR3C/RPC3 (3C); POLR3G/RPC7α (3G); POLR3GL/RPC7β (3GL); POLR3D/RPC4 (3D); POLR3E/RPC5 (3E); BRF1/TFIIIB90; TF3C1/TFIIIC220. Corresponding subcomplex indicated. ( c-k ) Example ChIP-seq read signals for each subunit/factor are shown in THP-1 monocytes across canonical Pol III-transcribed genes of varying promoter architecture, including ribosomal 5S rRNA genes (type 1 promoter; panel c ), tRNA, 7SL, vault, and SNAR RNA genes (type 2 promoter, panels d-g , respectively), and Y, U6, 7SK, and RMRP RNA genes (type 3 promoter, panels h-k , respectively). Gene labels include Unique RNA Sequence (URS) identifiers assigned by (and connected to) the RNAcentral database. Bottom panel illustrates corresponding promoter architecture classification. ( l ) Cartoon schematic of the Pol III protein complex with emphasis on subunits mapped in this study (labeled) and the corresponding color code for genomic signal plots. Illustration serves as general reference guide inspired by previous structural reconstructions (Hoffmann et al., Nature 2015) . ( m ) Scatterplot visualization of the correlation between normalized read densities for paralogous Pol III subunits, POLR3G and POLR3GL, at Pol III complex-occupied genes in THP-1 monocytes.

Article Snippet: 5 ug of antibody per ChIP was coupled to 18 uL of beads and rotated overnight with sheared chromatin at 4° C. Beads were then washed 5x in ChIP wash buffer (Santa Cruz), 1x in TE, and chromatin eluted in TE + 1% SDS.

Techniques: Disruption, Genome Wide, ChIP-sequencing, Sequencing, Labeling

( a ) Heatmap visualization of ChIP-seq signal density over individual Pol III-transcribed genes for Pol III subunits POLR3A, POLR3B, POLR1D, POLR3C, POLR3G, POLR3GL, POLR3D, and POLR3E, TFIIIB subunit BRF1, and TFIIIC subunit TF3C1. Heatmap is ordered by the median signal density across Pol III subunits over canonical Pol III-transcribed genes (n = top 1,000 canonical genes; RNAcentral annotation). Corresponding gene type indicated by right-flanking colorbar. ( b ) Heatmap visualization of Pearson correlation between chromatin IP experiments for individual Pol III subunits and BRF1, GTF3C1. ( c-h ) Correlation scatterplot visualization between Pol III subunit POLR3G and Pol III subunits POLR3A, POLR3B, POLR1D, POLR3C, POLR3D, and POLR3E, respectively. ( i ) Scatterplot visualization of correlation between Pol III subunit POLR3G signal enrichment and chromatin accessibility profile (ATAC-seq) at Pol III occupied genes in THP-1 monocytes. ( j ) Analogous correlation plot between chromatin accessibility profile (ATAC-seq) and median Pol III subunit signal enrichment. ( k ) Gene expression profile for mapped Pol III subunits in THP-1 cells (points represent individual biological replicates).

Journal: bioRxiv

Article Title: A cancer-associated RNA polymerase III identity drives robust transcription and expression of SNAR-A noncoding RNA

doi: 10.1101/2021.04.21.440535

Figure Lengend Snippet: ( a ) Heatmap visualization of ChIP-seq signal density over individual Pol III-transcribed genes for Pol III subunits POLR3A, POLR3B, POLR1D, POLR3C, POLR3G, POLR3GL, POLR3D, and POLR3E, TFIIIB subunit BRF1, and TFIIIC subunit TF3C1. Heatmap is ordered by the median signal density across Pol III subunits over canonical Pol III-transcribed genes (n = top 1,000 canonical genes; RNAcentral annotation). Corresponding gene type indicated by right-flanking colorbar. ( b ) Heatmap visualization of Pearson correlation between chromatin IP experiments for individual Pol III subunits and BRF1, GTF3C1. ( c-h ) Correlation scatterplot visualization between Pol III subunit POLR3G and Pol III subunits POLR3A, POLR3B, POLR1D, POLR3C, POLR3D, and POLR3E, respectively. ( i ) Scatterplot visualization of correlation between Pol III subunit POLR3G signal enrichment and chromatin accessibility profile (ATAC-seq) at Pol III occupied genes in THP-1 monocytes. ( j ) Analogous correlation plot between chromatin accessibility profile (ATAC-seq) and median Pol III subunit signal enrichment. ( k ) Gene expression profile for mapped Pol III subunits in THP-1 cells (points represent individual biological replicates).

Article Snippet: 5 ug of antibody per ChIP was coupled to 18 uL of beads and rotated overnight with sheared chromatin at 4° C. Beads were then washed 5x in ChIP wash buffer (Santa Cruz), 1x in TE, and chromatin eluted in TE + 1% SDS.

Techniques: ChIP-sequencing, Chromatin Immunoprecipitation, Gene Expression